2020/12/21 12:03:21
高通量組織研磨儀研磨腫瘤組織提取RNA
Source：Shanghai Jingxin
實驗目的
研磨破碎腫瘤組織細胞（如胰腺、肺、肝等器官的腫瘤組織），后續提取其RNA。
實驗原理
通過研磨珠撞擊腫瘤組織細胞，使其細胞膜破裂，其內的RNA暴露。
實驗材料和器具
樣品：腫瘤組織細胞
儀器：高通量組織研磨儀（上海凈信，Tissuelyser-48）
耗材：離心管，研磨珠（上海凈信）
實驗步驟
1.取組織塊解凍，用生理鹽水洗去血污，剪取少許組織，剪小放入離心管中；
2.加入研磨珠，加入TES混勻，再加入SDS，蛋白酶K，充分混勻；
3.離心管放入研磨機中，按要求設置好參數，程序結束即可得到破碎原液；
4.完成后續RNA提取步驟。
實驗結果
圖1 胰腺腫瘤組織RNA提取
Previous：高通量組織研磨儀對包涵體（大腸桿菌）蛋白質提取的研磨方法
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